cd73 inhibited apoevs Search Results


cd73 inhibited apoevs  (MedChemExpress)
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MedChemExpress cd73 inhibited apoevs
ApoEVs hydrolyze ATP to adenosine via surface CD39 and <t>CD73.</t> ( A ) The activity of ApoEVs and apoptotic T cells to hydrolyze ATP was measured in vitro (N = 3). ( B ) Extracellular ATP concentration in bone marrow plasma of sham and OVX animals (N = 6). ( C ) Western blot analysis of CD39 and CD73 in bone marrow of sham and OVX animals. ( D ) Extracellular adenosine concentration in bone marrow plasma of sham and OVX animals. ( E ) Western blot analysis of CD39 and CD73 on the membrane of apoptotic T cells and ApoEVs. ( F ) Immunoelectron microscopy detection of CD39 and CD73 on ApoEVs (scale bar = 200 nm). Yellow arrows indicate CD39 and red arrows indicate CD73 adhered by gold particles. ( G ) The activity of ApoEVs to hydrolyze ATP with or without POM (a CD39 inhibitor) or PSB (a CD73 inhibitor) was determined in vitro (N = 3). ( H ) The activity of ApoEVs in hydrolyzing ATP to adenosine with or without POM or PSB was determined in vitro (N = 3). Data are presented as mean ± SD; ns, not significant; ***P< 0.001 by one-way ANOVA with Tukey’s post hoc test or unpaired Student’s t test.
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ApoEVs hydrolyze ATP to adenosine via surface CD39 and CD73. ( A ) The activity of ApoEVs and apoptotic T cells to hydrolyze ATP was measured in vitro (N = 3). ( B ) Extracellular ATP concentration in bone marrow plasma of sham and OVX animals (N = 6). ( C ) Western blot analysis of CD39 and CD73 in bone marrow of sham and OVX animals. ( D ) Extracellular adenosine concentration in bone marrow plasma of sham and OVX animals. ( E ) Western blot analysis of CD39 and CD73 on the membrane of apoptotic T cells and ApoEVs. ( F ) Immunoelectron microscopy detection of CD39 and CD73 on ApoEVs (scale bar = 200 nm). Yellow arrows indicate CD39 and red arrows indicate CD73 adhered by gold particles. ( G ) The activity of ApoEVs to hydrolyze ATP with or without POM (a CD39 inhibitor) or PSB (a CD73 inhibitor) was determined in vitro (N = 3). ( H ) The activity of ApoEVs in hydrolyzing ATP to adenosine with or without POM or PSB was determined in vitro (N = 3). Data are presented as mean ± SD; ns, not significant; ***P< 0.001 by one-way ANOVA with Tukey’s post hoc test or unpaired Student’s t test.

Journal: International Journal of Nanomedicine

Article Title: T Cell-Derived Apoptotic Extracellular Vesicles Ameliorate Bone Loss via CD39 and CD73-Mediated ATP Hydrolysis

doi: 10.2147/IJN.S491222

Figure Lengend Snippet: ApoEVs hydrolyze ATP to adenosine via surface CD39 and CD73. ( A ) The activity of ApoEVs and apoptotic T cells to hydrolyze ATP was measured in vitro (N = 3). ( B ) Extracellular ATP concentration in bone marrow plasma of sham and OVX animals (N = 6). ( C ) Western blot analysis of CD39 and CD73 in bone marrow of sham and OVX animals. ( D ) Extracellular adenosine concentration in bone marrow plasma of sham and OVX animals. ( E ) Western blot analysis of CD39 and CD73 on the membrane of apoptotic T cells and ApoEVs. ( F ) Immunoelectron microscopy detection of CD39 and CD73 on ApoEVs (scale bar = 200 nm). Yellow arrows indicate CD39 and red arrows indicate CD73 adhered by gold particles. ( G ) The activity of ApoEVs to hydrolyze ATP with or without POM (a CD39 inhibitor) or PSB (a CD73 inhibitor) was determined in vitro (N = 3). ( H ) The activity of ApoEVs in hydrolyzing ATP to adenosine with or without POM or PSB was determined in vitro (N = 3). Data are presented as mean ± SD; ns, not significant; ***P< 0.001 by one-way ANOVA with Tukey’s post hoc test or unpaired Student’s t test.

Article Snippet: To prepare CD39- or CD73-inhibited ApoEVs, 30 μg/mL of ApoEVs were preincubated with 100 μM POM (dissolved in DMSO, MedChemExpress) or PSB (dissolved in water, MedChemExpress) for 40 minutes at room temperature.

Techniques: Activity Assay, In Vitro, Concentration Assay, Western Blot, Membrane, Immuno-Electron Microscopy

ApoEVs promote bone regeneration via surface CD39 and CD73. OVX mice were divided into four groups and injected with PBS, ApoEVs, and POM or PSB pretreated ApoEVs, respectively (N = 5–6). Extracellular ATP ( A ) and adenosine ( B ) concentration in bone marrow plasma in different groups of mice were detected. ( C ) Micro-CT analyses of trabecular bone mass in the femurs. ( D-G ) Quantitative analyses of BMD ( D ), BV/TV ( E ), Tb. N ( F ) and Tb. Sp ( G ). ( H-J ) ELISA assays of IFN-γ ( H ), IL-17 ( I) and TNF-α ( J ) concentrations in the serum of peripheral blood from indicated groups. Data are presented as mean ± SD; ns, not significant; *P< 0.05; **P< 0.01; ***P< 0.001 by one-way ANOVA with Tukey’s post hoc test.

Journal: International Journal of Nanomedicine

Article Title: T Cell-Derived Apoptotic Extracellular Vesicles Ameliorate Bone Loss via CD39 and CD73-Mediated ATP Hydrolysis

doi: 10.2147/IJN.S491222

Figure Lengend Snippet: ApoEVs promote bone regeneration via surface CD39 and CD73. OVX mice were divided into four groups and injected with PBS, ApoEVs, and POM or PSB pretreated ApoEVs, respectively (N = 5–6). Extracellular ATP ( A ) and adenosine ( B ) concentration in bone marrow plasma in different groups of mice were detected. ( C ) Micro-CT analyses of trabecular bone mass in the femurs. ( D-G ) Quantitative analyses of BMD ( D ), BV/TV ( E ), Tb. N ( F ) and Tb. Sp ( G ). ( H-J ) ELISA assays of IFN-γ ( H ), IL-17 ( I) and TNF-α ( J ) concentrations in the serum of peripheral blood from indicated groups. Data are presented as mean ± SD; ns, not significant; *P< 0.05; **P< 0.01; ***P< 0.001 by one-way ANOVA with Tukey’s post hoc test.

Article Snippet: To prepare CD39- or CD73-inhibited ApoEVs, 30 μg/mL of ApoEVs were preincubated with 100 μM POM (dissolved in DMSO, MedChemExpress) or PSB (dissolved in water, MedChemExpress) for 40 minutes at room temperature.

Techniques: Injection, Concentration Assay, Micro-CT, Enzyme-linked Immunosorbent Assay